Designing primers using amplifx7/15/2023 ![]() PrimerStation - multiplex human PCR primer design site.Primer Generator - Automated generator of primers for site-directed mutagenesis.Primer3Plus - Use primer3 to pick primers for specific tasks.Primer3 - a common used software for designing primers.Primaclade - a web-based application that accepts a multiple species nucleotide alignment file as input and identifies a set of PCR primers that will bind across the alignment.PRIDE - The less automated webversion of PRIDE (a.o.PCR suite - a collection of programs to search overlapping primers, genomic primers for exon amplification, SNP- and cDNA flanking primers.Osprey - Oligonucleotide Design Software for Sequencing and Gene Expression.The oligodb system uses the human DNA-transcripts of ENSEMBL. Oligodb - a web-based system for interactive design of oligo DNA for transcription profiling (hybridization) of human genes.NetPrimer - Free primer design service of Premier Biosoft. ![]() MutScreener - Design primers for mutation screening (by PCR-direct sequencing).Methprimer - Design primers for methylation PCR.MEDUSA - A tool for automatic selection and visual assessment of PCR primer pairs (Karolinska).Genefisher - Interactive PCR primer design.Exonprimer - Design primers for the amplification of exons with intronic primers.CODEHOP - COnsensus-DEgenerate Hybrid Oligonucleotide Primers.BatchPrimer3 - High throughput web application for PCR and sequencing primer design.AutoPrime - designs primers that are specific for expressed sequences (mRNA). ![]()
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